During our clinical trials, samples were collected from all recipient procedures in the UK and from all donor and recipient procedures on the continent. Sample collection included (depending on each of the trials) blood, urine, perfusate and bile samples as well as kidney and liver biopsies at different timepoints of organ retrieval and transplantation. Samples were processed, stored and transferred under standard operating procedures in dedicated state of the art bio-banking facilities in Oxford.

Our COPE bio-repository promotes translational research to identify novel biomarkers of organ quality that can predict graft function and survival after transplantation. The identification of proteomic and molecular signatures of organ injury will enable the monitoring of innovative interventions carried out in our three trials. Proteomic, metabolomic and molecular techniques will be deployed to elucidate mechanisms of ischemia / reperfusion injury and evaluate the preservation strategies used in the clinical trials.

Sample collection time points and sample types vary across our clinical trials and for more detailed information, please get in touch with the COPE project office. However, main time points include:

  • after organ retrieval
  • 15min after start of organ perfusion
  • after anaesthesia and before knife-to-skin time
  • at the end of organ preservation
  • 1 hour post-reperfusion or prior to closure (whichever comes first)

Samples are kept in the following formats:

  • Blood: plasma (EDTA) and serum (SST) stored at -80°C; aliquots of 0.5ml
  • Urine: centrifuged urine stored at -80°C; aliquots of 0.5ml
  • Perfusate: centrifuged perfusate from normothermic (liver) and hypothermic (kidney) perfusion stored at -80°C; aliquots of 0.5ml
  • Bile: centrifuged bile from normothermic liver perfusion stored at -80°C; aliquots of 1ml
  • Frozen tissue: kidney and liver biopsies stored in vapour phase liquid nitrogen (-196°C)
  • Fixed tissue: kidney and liver biopsies formalin fixed paraffin embedded tissue blocks

Samples are collected by help of our dedicated COPE sample collection boxes pre-packaged with the necessary tubes and barcodes for easy biobanking.

To access samples, research proposals can be submitted to COPE for review and approval. If you would like to work with COPE samples, please refer to the steps outlined in our research section.

See COPE Authorship Policy.

This project has received funding from the European Union's 7th Framework Programme for research, technological development and demonstration under grant agreement 305934.

COPE Consortium - 2018