Biobank

To access samples, research proposals can be submitted to COPE for review and approval. If you would like to work with COPE samples, pleease read the access policy below and refer to the steps outlined in our research section.

During our clinical trials, samples are collected from all recipient procedures in the UK and from all donor and recipient procedures on the continent. Sample collection includes blood, urine, perfusate and bile samples as well as kidney and liver biopsies at different timepoints of organ retrieval and transplantation. Samples are processed, stored and transferred under standard operating procedures in dedicated state of the art bio-banking facilities in Oxford.

 

Our COPE biobank promotes translational research to identify novel biomarkers of organ quality that can predict graft function and survival after transplantation. The identification of proteomic and molecular signatures of organ injury will enable the monitoring of innovative interventions carried out in our three trials. Proteomic, metabolomic and molecular techniques will be deployed to elucidate mechanisms of ischemia / reperfusion injury and evaluate the preservation strategies used in the clinical trials.

Samples are kept in the following formats:

 

  • Blood: plasma (EDTA) and serum (SST) stored at -80°C; aliquots of 0.5ml
  • Urine: centrifuged urine stored at -80°C; aliquots of 0.5ml
  • Perfusate: centrifuged perfusate from normothermic (liver) and hypothermic (kidney) perfusion stored at -80°C; aliquots of 0.5ml
  • Bile: centrifuged bile from normothermic liver perfusion stored at -80°C; aliquots of 0.5ml
  • Frozen tissue: kidney and liver biopsies stored in vapour phase liquid nitrogen (-196°C)
  • Fixed tissue: kidney and liver biopsies formalin fixed paraffin embedded tissue blocks

Sample collection time points vary across our clinical trials and for more detailed information, please get in touch with the COPE project office. However, main time points include: after organ retrieval; 15min after start of organ perfusion; at end of organ preservation and 1 hour post-reperfusion.

Samples are collected by help of our dedicated COPE sample collection boxes pre-packaged with the necessary tubes and barcodes for easy biobanking.

This project has received funding from the European Union's 7th Framework Programme for research, technological development and demonstration under grant agreement 305934.

COPE Consortium - 2017